Peripheral Blood

3 ml sodium heparin (green top) - preferred or
3 ml in EDTA (purple top)

Bone Marrow Aspirate  

1-2 ml sodium heparin (green top) - preferred or
1-2 ml in EDTA (purple top)

Tissue

Multiple 2-3 mm pieces in RPMI transport media
(optimum RPMI to tissue ratio is 15:1; multiple vials accepted)

Body Fluids

Mix 1:1 in RPMI transport media

PNH Profile

3 ml peripheral blood in EDTA (purple top) - preferred or
3 ml peripheral blood in sodium heparin (green top)
(must be processed within 24 hrs of collection) 

Peripheral Blood

5 ml in sodium heparin (green top)

Bone Marrow Aspirate  

1-2 ml in sodium heparin (green top)

Cord Blood

2-5 ml in sodium heparin (green top)

Tissue

Multiple 2-3 mm pieces in RPMI transport media
(optimum RPMI to tissue ratio is 15:1; multiple vials accepted)

Peripheral Blood

3 ml sodium heparin (green top) - preferred or
3 ml in EDTA (purple top)

Bone Marrow Aspirate  

1-2 ml sodium heparin (green top) - preferred or
3 ml in EDTA (purple top)

Tissue

Multiple 2-3 mm pieces in RPMI transport media
(optimum RPMI to tissue ratio is 15:1; multiple vials accepted

Formalin-fixed paraffin-embedded tissue

Peripheral Blood

5 ml EDTA (purple top) – preferred or
ACD (yellow top) acceptable 

Bone Marrow Aspirate  

1-2 ml EDTA (purple top) – preferred or
sodium heparin (green top) or ACD (yellow top) acceptable 

Tissue

Minimum of 250 mg tissue in RPMI transport media 

Formalin-fixed paraffin-embedded tissue