Test Method
FISH
Disease Long Name
Acute lymphoblastic leukemia
Disease Abbreviation
ALL
CPT Code
88377
Probes / Test Description
CRLF2-Breakapart, ABL2 (1q25.2), PDGFRB-Breakapart, JAK2-Breakapart, ABL1, CSFR1 (5q32), EPOR (19p13.2)
Specimen Requirements
- Bone marrow aspirate: 2-3 mL in sodium heparin (green top) preferred, or 1-2 mL in EDTA (purple top)
- Peripheral blood: 3 mL in sodium heparin (green top) preferred, or 3 mL in EDTA (purple top)
- Fresh Tissue: Multiple 2-3 mm pieces of tissue in RPMI transport media (optimum RPMI to tissue ratio is 15:1; (multiple vials are acceptable)
- Formalin-Fixed Paraffin-Embedded Tissue: Minimum 0.2 x 0.2 x 0.2 cm tissue, non-decalcified tissue only
Storage & Transport
Ship specimen the same day whenever possible, with refrigerated (not frozen) cold pack.
Label all tubes with at least two unique patient identifiers. Slides require one patient identifier. Identifiers include name, DOB, specimen ID# or medical record#.
Label all tubes with at least two unique patient identifiers. Slides require one patient identifier. Identifiers include name, DOB, specimen ID# or medical record#.
Turnaround time
3 - 5 days
Level of Service
Global
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