Test Method
FISH
Disease Long Name
Follicular Lymphoma, Non-Hodgkin's Lymphoma
Disease Abbreviation
FL, FCL, NHL
CPT Code
88377x3 (Global)
88374x3 (Tech-only)
88374x3 (Tech-only)
Probes / Test Description
t(14;18)/IGH-BCL2, BCL6-break-apart, BCL2-break-apart
IF GAIN IGH, REFLEX: [t(8;14)/IGH-MYC, MYC-break-apart]
IF question of MCL by Flow, REFLEX: [t(6:14)/CCND3-IGH, t(11;14)/CCND1-IGH, CCND1-break-apart, CCND2-break-apart]
IF GAIN IGH, REFLEX: [t(8;14)/IGH-MYC, MYC-break-apart]
IF question of MCL by Flow, REFLEX: [t(6:14)/CCND3-IGH, t(11;14)/CCND1-IGH, CCND1-break-apart, CCND2-break-apart]
Specimen Requirements
- Bone marrow aspirate: 2-3 mL in sodium heparin (green top) preferred, or 1-2 mL in EDTA (purple top)
- Peripheral blood: 3 mL in sodium heparin (green top) preferred, or 3 mL in EDTA (purple top)
- Fresh Tissue: Multiple 2-3 mm pieces of tissue in RPMI transport media (optimum RPMI to tissue ratio is 15:1; (multiple vials are acceptable)
- Formalin-Fixed Paraffin-Embedded Tissue: Minimum 0.2 x 0.2 x 0.2 cm tissue, non-decalcified tissue only
Storage & Transport
Ship specimen the same day whenever possible, with refrigerated (not frozen) cold pack.
Label all tubes with at least two unique patient identifiers. Slides require one patient identifier. Identifiers include name, DOB, specimen ID# or medical record#.
Label all tubes with at least two unique patient identifiers. Slides require one patient identifier. Identifiers include name, DOB, specimen ID# or medical record#.
Turnaround time
3 - 5 days
Level of Service
Global
Tech-Only
Tech-Only