Test Method
FISH
Disease Long Name
Marginal Zone Lymphoma
Disease Abbreviation
MZL
CPT Code
88377x5, 88368 (Global)
88374x5, 88367 (Tech-only)
88374x5, 88367 (Tech-only)
Probes / Test Description
7q/CEP7, CEP12, BCL6-break-apart, MALT1-break-apart, IGH-break-apart, TP53/CEP17
IF MALT1-break-apart rearranged, REFLEX TO: t(11;18)/BIRC3-MALT1, t(14;18)/IGH-MALT1
IF IGH-break-apart rearranged, REFLEX TO: t(11;14)/CCND1-IGH, CCND1-break-apart, t(14;18)/IGH-BCL2
IF unresolved question of MCL, REFLEX: t(6:14)/CCND3-IGH, CCND2-break-apart
IF MALT1-break-apart rearranged, REFLEX TO: t(11;18)/BIRC3-MALT1, t(14;18)/IGH-MALT1
IF IGH-break-apart rearranged, REFLEX TO: t(11;14)/CCND1-IGH, CCND1-break-apart, t(14;18)/IGH-BCL2
IF unresolved question of MCL, REFLEX: t(6:14)/CCND3-IGH, CCND2-break-apart
Specimen Requirements
- Bone marrow aspirate: 2-3 mL in sodium heparin (green top) preferred, or 1-2 mL in EDTA (purple top)
- Peripheral blood: 3 mL in sodium heparin (green top) preferred, or 3 mL in EDTA (purple top)
- Fresh Tissue: Multiple 2-3 mm pieces of tissue in RPMI transport media (optimum RPMI to tissue ratio is 15:1; (multiple vials are acceptable)
- Formalin-Fixed Paraffin-Embedded Tissue: Minimum 0.2 x 0.2 x 0.2 cm tissue, non-decalcified tissue only
Storage & Transport
Ship specimen the same day whenever possible, with refrigerated (not frozen) cold pack.
Label all tubes with at least two unique patient identifiers. Slides require one patient identifier. Identifiers include name, DOB, specimen ID# or medical record#.
Label all tubes with at least two unique patient identifiers. Slides require one patient identifier. Identifiers include name, DOB, specimen ID# or medical record#.
Turnaround time
3 - 5 days
Level of Service
Global
Tech-Only
Tech-Only