Test Method

Flow Cytometry

Disease / Condition

Mast Cell Leukemia

Disease Abbreviation


CPT Codes

88185x2 when reflexed from tissue of fluid; 88185x1 when reflexed from bone marrow or peripheral blood

Test Description

CD2, CD25, CD45, CD117, Near-IR

Specimen Requirements

  • Bone marrow aspirate: 1-2 mL in sodium heparin (green top) preferred, or 1-2 mL in EDTA (purple top)
  • Peripheral blood: 3 mL in sodium heparin (green top) preferred, or 3 mL in EDTA (purple top)
  • Fresh Tissue: Multiple 2-3 mm pieces of tissue in RPMI transport media (optimum RPMI to tissue ratio is 15:1; (multiple vials are acceptable)
  • Body Fluids: Mix 1:1 in RPMI transport media

Storage & Transport

Ship specimen the same day whenever possible, with refrigerated (not frozen) cold pack.

Label all tubes with at least two unique patient identifiers. Slides require one patient identifier. Identifiers include name, DOB, specimen ID# or medical record.

Turnaround time

24 hours

Level of Service