Test Method

Flow Cytometry

Disease / Condition

B-cell Acute Lymphoblastic Leukemia

Disease Abbreviation


CPT Codes

88185x10; dependent on initial limited panel.

Test Description

CD3, CD10, CD19, CD22, CD34, CD38, CD45, Kappa, Lambda, Near-IR

Specimen Requirements

  • Bone marrow aspirate: 1-2 mL in sodium heparin (green top) preferred, or 1-2 mL in EDTA (purple top)
  • Peripheral blood: 3 mL in sodium heparin (green top) preferred, or 3 mL in EDTA (purple top)
  • Fresh Tissue: Multiple 2-3 mm pieces of tissue in RPMI transport media (optimum RPMI to tissue ratio is 15:1; (multiple vials are acceptable)
  • Body Fluids: Mix 1:1 in RPMI transport media

Storage & Transport

Ship specimen the same day whenever possible, with refrigerated (not frozen) cold pack.

Label all tubes with at least two unique patient identifiers. Slides require one patient identifier. Identifiers include name, DOB, specimen ID# or medical record#.

Turnaround time

24 hours

Level of Service