Test Method

IHC

CPT Code

88342 x 1
88341 x 1

Stains / Test Description

Antibody specificity was demonstrated by detection of differential expression of the target protein in cell line upon treatment with secretion blockers. This would enable retention of target protein inside the cell. Relative expression of Chromogranin A was observed in SH-SY5Y and IMR-32 cells treated with PTI (1X for 4hr) and with FL1-06 (10uM for 4hr) as compared to the corresponding untreated cells using immunohistochemistry (Paraffin) analysis of Chromogranin A was performed in paraffin-embedded mouse pancreas tissue using Chromogranin A Polyclonal Antibody

Specimen Requirements

(1) H&E slide with formalin-fixed, paraffin-embedded (FFPE) tissue, fixed with 10% NBF.
or
One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4 microns) for each test/antibody ordered

Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.

Storage & Transport

Ship specimen same day (whenever possible) with refrigerated (not frozen) cold pack

Turnaround time

1-3 days depending on level of service

Level of Service

Global (with interpretation)
Slide Only (glass slides only)
Web-Enabled (glass and digital images through eCSI portal)