Test Method


CPT Code

88342 x 1
88341 x 1

Stains / Test Description

This cytokeratin cocktail can be useful in differentiation of the squamous cell carcinomas and adenocarcinomas. The 34bE12 clone recognizes cytokeratin (CK) 1, 5, 10 and 14. It is reactive with stratified epithelia, myoepithelial and basal cells of prostate and breast, and it stains squamous carcinomas and adenosquamous carcinomas (brown). Clone 5D3 recognizes cytokeratin (CK) 8 and 18 of all simple and glandular epithelium and adenocarcinomas (red).

Specimen Requirements

(1) H&E slide with formalin-fixed, paraffin-embedded (FFPE) tissue, fixed with 10% NBF.
One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4 microns) for each test/antibody ordered

Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.

Storage & Transport

Ship specimen same day (whenever possible) with refrigerated (not frozen) cold pack

Turnaround time

1-3 days depending on level of service

Level of Service

Global (with interpretation)
Slide Only (glass slides only)
Web-Enabled (glass and digital images through eCSI portal)