Test Method


CPT Code

88342 x 1
88341 x 1

Stains / Test Description

E-Cadherin is an adhesion protein that is expressed in cells of epithelial lineage. Anti-E-Cadherin stains positively in glandular epithelium as well as adenocarcinomas of the lung, G.I. tract, and ovary. It has been useful in distinguishing adenocarcinoma from mesothelioma. Another application involves the differentiation of ductal (which stains positive) vs. lobular cancer of the breast. It has also been shown to be positive in some thyroid carcinomas. Loss of E-Cadherin expression has been suggested as a poor prognostic sign in breast carcinoma and non-small cell lung carcinoma.

Specimen Requirements

(1) H&E slide with formalin-fixed, paraffin-embedded (FFPE) tissue, fixed with 10% NBF.
One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4 microns) for each test/antibody ordered

Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.

Storage & Transport

Ship specimen same day (whenever possible) with refrigerated (not frozen) cold pack

Turnaround time

1-3 days depending on level of service

Level of Service

Global (with interpretation)
Slide Only (glass slides only)
Web-Enabled (glass and digital images through eCSI portal)