Test Method


CPT Code

"88342 x 1
88341 x 1"

Stains / Test Description

Anti-Fascin is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells and myeloid cells. Anti-Fascin is positive in dendritic cells. This marker may be helpful in distinguishing between Hodgkin's disease and non-Hodgkin's lymphoma in difficult cases. Also, the lack of expression of fascin in the neoplastic follicles in follicular lymphoma can be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Anti-Fascin has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung, as well as ovarian cancer.

Specimen Requirements

(1) H&E slide with formalin-fixed, paraffin-embedded (FFPE) tissue, fixed with 10% NBF.
One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4 microns) for each test/antibody ordered

Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.

Storage & Transport

Ship specimen same day (whenever possible) with refrigerated (not frozen) cold pack

Turnaround time

1-3 days depending on level of service

Level of Service

"Global (with interpretation)
Slide Only (glass slides only)
Web-Enabled (glass and digital images through eCSI portal)"