Test Method


CPT Code

88342 x 1
88341 x 1

Stains / Test Description

This antibody is directed against purified bovine eye lens vimentin and has been known to react specifically with human vimentin. This reagent should be used with a panel of antibodies to aid in the immunohistochemical identification of cells of normal and abnormal mesenchymal lineage as an aid in diagnosis of anaplastic tumors in formalin fixed, paraffin embedded tissue. Anti-Vimentin (Vim 3B4) binds specifically with the 57kD intermediate filament protein, vimentin. The clinical interpretation of any staining, or the absence of staining, must be complemented by morphological studies.

Specimen Requirements

(1) H&E slide with formalin-fixed, paraffin-embedded (FFPE) tissue, fixed with 10% NBF.
One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4 microns) for each test/antibody ordered

Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.

Storage & Transport

Ship specimen same day (whenever possible) with refrigerated (not frozen) cold pack

Turnaround time

1-3 days depending on level of service

Level of Service

Global (with interpretation)
Slide Only (glass slides only)
Web-Enabled (glass and digital images through eCSI portal)