Test Method


CPT Codes

88377x4, 88368 (Global)
88374x4, 88367 (Tech-only)

Test Description

FLT3/IDH1/IDH2 *IF CYTO & FLT3 are Normal, REFLEX TO: CEBPA/NPM1 *IF FLT3(+) with monocytic differentiation, REFLEX TO: NPM1 *IF inv(16) or t(8;21), REFLEX TO: KIT exons 8,17

Specimen Requirements

  • Bone marrow aspirate: 2-3 mL in sodium heparin (green top) preferred, or 1-2 mL in EDTA (purple top)
  • Peripheral blood: 3 mL in sodium heparin (green top) preferred, or 3 mL in EDTA (purple top)
  • Fresh Tissue: Multiple 2-3 mm pieces of tissue in RPMI transport media (optimum RPMI to tissue ratio is 15:1; (multiple vials are acceptable)
  • Formalin-Fixed Paraffin-Embedded Tissue: 1 H&E slide cut to 4 microns and 6-8 slides cut at 8 microns of BM clot or tissue fixed with 10% NBF fixative on non-charged slides. Please circle tumor for microdissection. Alternatively, the FFPE clock of the BM clot can be sent for sectioning in our lab.

Storage & Transport

Ship specimen the same day whenever possible, with refrigerated (not frozen) cold pack.

Label all tubes with at least two unique patient identifiers. Slides require one patient identifier. Identifiers include name, DOB, specimen ID# or medical record .

Turnaround time

3 - 5 days

Level of Service