Test Method


CPT Codes


Test Description

Test uses PCR and fragment analysis for quantitative detection of IGH-BCL2 translocations, which are associated with 70-80% of follicular lymphoma and approximately 20% of diffuse large B-cell lymphoma. Identifies translocations involving the major (MBR), minor (MCR), and 3' MBR sub-cluster regions of BCL2. Positive results quantify the ratio of mutant BCL2 to internal control DNA. this test is useful to confirm the diagnosis of follicular lymphoma, monitor effectiveness of treatment, and to detect minimal residual disease or recurrence.

Specimen Requirements

  • Bone marrow aspirate: 1-2 mL in EDTA tube (purple top) preferred; ACD (yellow top) acceptable
  • Peripheral blood: 5-10 mL in EDTA tube (purple top) preferred; ACD (yellow top) acceptable
  • Fresh Tissue: Minimum of 250 mg tissue in RPMI transport media
  • Formalin-Fixed Paraffin-Embedded Tissue: 1 H&E slide cut to 4 microns and 6-8 slides cut at 8 microns of BM clot or tissue fixed with 10% NBF fixative on non-charged slides. Please circle tumor for microdissection. Alternatively, the FFPE clock of the BM clot can be sent for sectioning in our lab.

Storage & Transport

Ship specimen the same day whenever possible, with refrigerated (not frozen) cold pack.

Label all tubes with at least two unique patient identifiers. Slides require one patient identifier. Identifiers include name, DOB, specimen ID# or medical record#.

Turnaround time

5 - 7 days

Level of Service